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Western blot analysis of extracts from Mouse heart tissue lyaste.using GLUT4 Mouse mAb [Klip] at dilution of 1:1000 incubated at 4℃ over night
Protein names :insulin-responsive; Glucose transporter GLUT 4; Glucose transporter type 4; Glucose transporter type 4 insulin responsive;GLUT4; Insulin responsive glucose transporter type 4;SLC2A4 ;Solute carrier family 2 member 4;
UniProtID :P14672
MASS(da) :54,787
MW(kDa) :50kD
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Rat,Mouse
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human GLUT4 .
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Mouse heart tissue lyaste
Function : Insulin-regulated facilitative glucose transporter, which plays a key role in removal of glucose from circulation. Response to insulin is regulated by its intracellular localization: in the absence of insulin, it is efficiently retained intracellularly within storage compartments in muscle and fat cells. Upon insulin stimulation, translocates from these compartments to the cell surface where it transports glucose from the extracellular milieu into the cell..
Tissue specificity :Skeletal and cardiac muscles,brown and white fat.
Subcellular locationi :Cell membrane,Multi-pass membrane protein. Endomembrane system,Multi-pass membrane protein. Cytoplasm, perinuclear region.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.