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Western blot analysis of extracts from MCF-7 cells lyaste.using PSMD1 Rabbit mAb [P111] at dilution of 1:1000 incubated at 4℃ over night
Protein names :PSMD1,PSMD1_HUMAN,26S proteasome regulatory subunit RPN2,26S proteasome regulatory subunit S1,26S proteasome subunit p112
UniProtID :Q99460
MASS(da) :105,836
MW(kDa) :106kDa
Form :Liquid
Purification :Protein A affinity purified.
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human Mouse Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human PSMD1
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :MCF-7 cells
Function :Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair.
Subcellular locationi :Proteasome
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.