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PDHA1 Rabbit mAb [X5A3]Cat NO.: A75884

Western blot(SDS PAGE) analysis of extracts from A549 cells.Using PDHA1Rabbit mAb [X5A3] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :PDHA1,PHE1A,ODPA_HUMAN,Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial

UniProtID :P08559

MASS(da) :43,296

MW(kDa) :43 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • 免疫荧光(ICC/IF) 1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human PDHA1

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :A549 cells

Function : The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle..

Tissue specificity :Ubiquitous.

Subcellular locationi :Mitochondrion matrix.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.