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Western blot(SDS PAGE) analysis of extracts from Jurkat cells.Using Superoxide Dismutase 1Rabbit mAb [OR09] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :SOD1,SODC_HUMAN,Superoxide dismutase [Cu-Zn]
UniProtID :P00441
MASS(da) :15,936
MW(kDa) :18 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Superoxide Dismutase 1
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Jurkat cells
Function : Destroys radicals which are normally produced within the cells and which are toxic to biological systems..
Subcellular locationi :Cytoplasm. Mitochondrion. Nucleus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.