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Western blot(SDS PAGE) analysis of extracts from HEK-293 cells.Using Clathrin light chainRabbit mAb [26PX] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :CLTA,CLCA_HUMAN,Clathrin light chain A
UniProtID :P09496
MW(kDa) :35 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Clathrin light chain
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected : HEK-293 cells
Function : Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Acts as component of the TACC3/ch-TOG/clathrin complex proposed to contribute to stabilization of kinetochore fibers of the mitotic spindle by acting as inter-microtubule bridge (PubMed:15858577, PubMed:21297582)..
Subcellular locationi :Cytoplasmic vesicle membrane,Peripheral membrane protein,Cytoplasmic side. Membrane, coated pit,Peripheral membrane protein,Cytoplasmic side. Cytoplasm, cytoskeleton, spindle.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.