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Western blot(SDS PAGE) analysis of extracts from HeLa cells .Using PFKFB3Rabbit mAb [6J3O] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :PFKFB1,F6PK,PFRX,F261_HUMAN,6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 1 [Includes: 6-phosphofructo-2-kinase ,Fructose-2,6-bisphosphatase ]
UniProtID :P16118,Q16877
MASS(da) :54,681
MW(kDa) :58 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human PFKFB3
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells
Function : Synthesis and degradation of fructose 2,6-bisphosphate..
Tissue specificity :Liver.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.