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Western blot(SDS PAGE) analysis of extracts from Jurkat cells.Using BCAT1 Rabbit mAb [L1EW] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :BCAT1,BCT1,ECA39,BCAT1_HUMAN,Branched-chain-amino-acid aminotransferase, cytosolic )
UniProtID :P54687
MASS(da) :42,966
MW(kDa) :43 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of Human BCAT1
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Jurkat cells
Function : Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine.
Tissue specificity :During embryogenesis, expressed in the brain and kidney. Overexpressed in MYC-induced tumors such as Burkitt's lymphoma.
Subcellular locationi :Cytoplasm.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.