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Western blot(SDS PAGE) analysis of extracts from HAP1 cells .Using Cyclin E1Rabbit mAb [31VT] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :CCNE1,CCNE,CCNE1_HUMAN,G1/S-specific cyclin-E1
UniProtID :P24864
MASS(da) :47,077
MW(kDa) :50 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Cyclin E1
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HAP1 cells
Function : Essential for the control of the cell cycle at the G1/S (start) transition..
Tissue specificity :Highly expressed in testis and placenta. Low levels in bronchial epithelial cells..
Subcellular locationi :Nucleus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.