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Western blot(SDS PAGE) analysis of extracts from K562 cells.Using Methylmalonyl Coenzyme A mutaseRabbit mAb [948R] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :MMUT,MUT,MUTA_HUMAN,Methylmalonyl-CoA mutase, mitochondrial
UniProtID :P22033
MASS(da) :83,134
MW(kDa) :83 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Methylmalonyl Coenzyme A mutase
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :K562 cells
Function : Catalyzes the reversible isomerization of methylmalonyl-CoA (MMCoA) (generated from branched-chain amino acid metabolism and degradation of dietary odd chain fatty acids and cholesterol) to succinyl-CoA (3-carboxypropionyl-CoA), a key intermediate of the tricarboxylic acid cycle..
Subcellular locationi :Mitochondrion matrix. Mitochondrion. Cytoplasm.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.