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Western blot(SDS PAGE) analysis of extracts from HEK-293T cells.Using Cytochrome P450 ReductaseRabbit mAb [N849] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :POR,CYPOR,NCPR_HUMAN,NADPH--cytochrome P450 reductase
UniProtID :P16435
MASS(da) :76,690
MW(kDa) :80 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Cytochrome P450 Reductase
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HEK-293T cells
Function : This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5..
Subcellular locationi :Endoplasmic reticulum membrane,Single-pass membrane protein,Cytoplasmic side.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.