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Western blot(SDS PAGE) analysis of extracts from HepG2 cells.Using Desmoglein 2/DSG2Rabbit mAb [TJIB] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :DSG2,CDHF5,DSG2_HUMAN,Desmoglein-2
UniProtID :Q14126
MASS(da) :122,294
MW(kDa) :150 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Desmoglein 2/DSG2
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HepG2 cells
Function : Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion.
Tissue specificity :All of the tissues tested and carcinomas.
Subcellular locationi :Cell membrane,Single-pass type I membrane protein. Cell junction, desmosome.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.