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Western blot(SDS PAGE) analysis of extracts from HepG2 cells.Using PON2Rabbit mAb [2588] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :PON2,PON2_HUMAN,Serum paraoxonase/arylesterase 2
UniProtID :Q15165
MASS(da) :39,381
MW(kDa) :39,41 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human PON2
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HepG2 cells
Function : Capable of hydrolyzing lactones and a number of aromatic carboxylic acid esters. Has antioxidant activity. Is not associated with high density lipoprotein. Prevents LDL lipid peroxidation, reverses the oxidation of mildly oxidized LDL, and inhibits the ability of MM-LDL to induce monocyte chemotaxis..
Tissue specificity :Widely expressed with highest expression in liver, lung, placenta, testis and heart..
Subcellular locationi :Membrane,Peripheral membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.