Cleaved Caspase-9 (Asp315) Rabbit pAbCat NO.: A65351

Protein names ：CASP9,MCH6,CASP9_HUMAN,Caspase-9 [Cleaved into: Caspase-9 subunit p35,Caspase-9 subunit p10]

UniProtID ：P55211

MASS(da) ：46,281

MW(kDa) ：35 kDa

Form ：Liquid

Purification ：peptide affinity chromatography

Host ：Rabbit

Isotype ：IgG

sensitivity ：Endogenous

Reactivity ：Human

• ApplicationDilution
• 免疫印迹(WB)1:1000-2000
• The optimal dilutions should be determined by the end user

Specificity ：Antibody is produced by immunizing animals with a synthetic peptide at the sequence of Human Cleaved Caspase-9 (Asp315)

Storage ：Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected ：HeLa cells staurosporine-treated (1 µM)

Function ： Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP)., Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.

Tissue specificity ：Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes..

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.