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Western blot(SDS PAGE) analysis of extracts from K-562 cells.Using CCTα Rabbit mAb [7I5I] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :PCYT1A,CTPCT,PCYT1,PCY1A_HUMAN,Choline-phosphate cytidylyltransferase A
UniProtID :P49585
MASS(da) :41,731
MW(kDa) :42 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of Human CCTα
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :K-562 cells
Function : Catalyzes the key rate-limiting step in the CDP-choline pathway for phosphatidylcholine biosynthesis..
Tissue specificity :Brain, placenta, liver, fetal and adult lung..
Subcellular locationi :Cytoplasm, cytosol. Membrane,Peripheral membrane protein. Endoplasmic reticulum. Nucleus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.