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Histone H4 Rabbit mAb [Y321]Cat NO.: A39889

Western blot(SDS PAGE) analysis of extracts from  NIH/3T3 cells .Using Histone H4Rabbit mAb [Y321] at dilution of 1:1000 incubated at 4℃ over night.

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订单 Datasheet

Product information

Protein names :H4C1,H4/A,H4FA,HIST1H4A,H4C2,H4/I,H4FI,HIST1H4B,H4C3,H4/G,H4FG,HIST1H4C,H4C4,H4/B,H4FB,HIST1H4D,H4C5,H4/J,H4FJ,HIST1H4E,H4C6,H4/C,H4FC,HIST1H4F,H4C8,H4/H,H4FH,HIST1H4H,H4C9,H4/M,H4FM,HIST1H4I,H4C11,H4/E,H4FE,HIST1H4J,H4C12,H4/D,H4FD,HIST1H4K,H4C13,H4/K,H4

UniProtID :P62805

MASS(da) :11,367

MW(kDa) :11 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • 免疫荧光(ICC/IF) 1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Histone H4

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected : NIH/3T3 cells 

Function : Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Subcellular locationi :Nucleus. Chromosome.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.