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Western blot(SDS PAGE) analysis of extracts from A375 cells.Using CD109 Rabbit mAb [2922] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :CD109,CPAMD7,CD109_HUMAN,CD109 antigen
UniProtID :Q6YHK3
MASS(da) :161,689
MW(kDa) :190 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of Human CD109
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :A375 cells
Function : Modulates negatively TGFB1 signaling in keratinocytes..
Tissue specificity :Widely expressed with high level in uterus, aorta, heart, lung, trachea, placenta and in fetal heart, kidney, liver, spleen and lung. Expressed by CD34(+) acute myeloid leukemia cell lines, T-cell lines, activated T-lymphoblasts, endothelial cells and activated platelets. Isoform 4 is expressed in placenta. Isoform 1 is expressed in keratinocytes and placenta..
Subcellular locationi :Cell membrane,Lipid-anchor, GPI-anchor.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.