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Western blot(SDS PAGE) analysis of extracts from NIH/3T3 cells.Using cdc25A Rabbit pAb at dilution of 1:1000 incubated at 4℃ over night.
Protein names :CDC25A,MPIP1_HUMAN,M-phase inducer phosphatase 1
UniProtID :P30304
MASS(da) :59,087
MW(kDa) :70 kDa
Form :Liquid
Purification :peptide affinity chromatography
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of Human cdc25A
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :NIH/3T3 cells
Function : Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDK1 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.