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Western blot(SDS PAGE) analysis of extracts from NIH/3T3 cells.Using AIDARabbit mAb [6JWU] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :AIDA,C1orf80,AIDA_HUMAN,Axin interactor, dorsalization-associated protein
UniProtID :Q96BJ3
MASS(da) :35,023
MW(kDa) :36 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human AIDA
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :NIH/3T3 cells
Function : Acts as a ventralizing factor during embryogenesis. Inhibits axin-mediated JNK activation by binding axin and disrupting axin homodimerization. This in turn antagonizes a Wnt/beta-catenin-independent dorsalization pathway activated by AXIN/JNK-signaling (By similarity)..
Tissue specificity :Widely expressed in adult tissues, with highest expression in the heart and skeletal muscle..
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.