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Western blot(SDS PAGE) analysis of extracts from HEK-293T cells transfected with mouse GMEB1.Using GMEB1Rabbit mAb [X212] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :GMEB1,GMEB1_HUMAN,Glucocorticoid modulatory element-binding protein 1
UniProtID :Q9Y692
MASS(da) :62,591
MW(kDa) :82 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human GMEB1
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HEK-293T cells transfected with mouse GMEB1
Function : Trans-acting factor that binds to glucocorticoid modulatory elements (GME) present in the TAT (tyrosine aminotransferase) promoter and increases sensitivity to low concentrations of glucocorticoids. Binds also to the transferrin receptor promoter. Essential auxiliary factor for the replication of parvoviruses.
Subcellular locationi :Nucleus. Cytoplasm.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.