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Western blot(SDS PAGE) analysis of extracts from Mouse brain.Using CNPase Rabbit pAb at dilution of 1:1000 incubated at 4℃ over night.
Protein names :CNP,CN37_HUMAN,2',3'-cyclic-nucleotide 3'-phosphodiesterase
UniProtID :P09543
MASS(da) :47,579
MW(kDa) :47 kDa
Form :Liquid
Purification :peptide affinity chromatography
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of Human CNPase
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Mouse brain
Function : Catalyzes the formation of 2'-nucleotide products from 2',3'-cyclic substrates (By similarity). May participate in RNA metabolism in the myelinating cell, CNP is the third most abundant protein in central nervous system myelin (By similarity)..
Subcellular locationi :Membrane,Lipid-anchor. Melanosome.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.