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Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using Coilin Rabbit mAb [Q0D0] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :COIL,CLN80,COIL_HUMAN,Coilin
UniProtID :P38432
MASS(da) :62,608
MW(kDa) :80 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of Human Coilin
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells
Function : Component of nuclear coiled bodies, also known as Cajal bodies or CBs, which are involved in the modification and assembly of nucleoplasmic snRNPs..
Tissue specificity :Found in all the cell types examined.
Subcellular locationi :Nucleus. Nucleus, Cajal body.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.