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CHD4 Rabbit mAb [TR0A]Cat NO.: A49016

Western blot(SDS PAGE) analysis of extracts from NCCIT cells.Using CHD4Rabbit mAb [TR0A] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :CHD4,CHD4_HUMAN,Chromodomain-helicase-DNA-binding protein 4

UniProtID :Q14839

MASS(da) :218,005

MW(kDa) :280 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫荧光(ICC/IF) 1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human CHD4

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :NCCIT cells

Function : Component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones..

Subcellular locationi :Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.