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Western blot(SDS PAGE) analysis of extracts from MCF-7 cells.Using DEPTOR/DEPDC6 Rabbit mAb [3E27] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :DEPTOR,DEPDC6,DPTOR_HUMAN,DEP domain-containing mTOR-interacting protein
UniProtID :Q8TB45
MASS(da) :46,294
MW(kDa) :48 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of Human DEPTOR/DEPDC6
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :MCF-7 cells
Function : Negative regulator of the mTORC1 and mTORC2 signaling pathways. Inhibits the kinase activity of both complexes..
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.