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Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using P-DUSP1/MKP1 (S359) Rabbit mAb [GJ53] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :DUSP1,CL100,MKP1,PTPN10,VH1,DUS1_HUMAN,Dual specificity protein phosphatase 1
UniProtID :P28562
MASS(da) :39,298
MW(kDa) :40 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of Human Phospho-DUSP1/MKP1 (Ser359)
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells
Function : Dual specificity phosphatase that dephosphorylates MAP kinase MAPK1/ERK2 on both 'Thr-183' and 'Tyr-185', regulating its activity during the meiotic cell cycle..
Tissue specificity :Expressed at high levels in the lung, liver placenta and pancreas. Moderate levels seen in the heart and skeletal muscle. Lower levels found in the brain and kidney..
Subcellular locationi :Nucleus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.