* 产品详情- */>
Western blot(SDS PAGE) analysis of extracts from PC-3 cells .Using P-EIF4B (S406) Rabbit mAb [MDA0] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :EIF4B,IF4B_HUMAN,Eukaryotic translation initiation factor 4B
UniProtID :P23588
MASS(da) :69,151
MW(kDa) :70 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human P-EIF4B (S406).
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 51% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :PC-3 cells
Function : Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.