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Western blot(SDS PAGE) analysis of extracts from Raw 264.7 cells.Using F4/80 Rabbit mAb [E7SN] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :ADGRE1,EMR1,TM7LN3,AGRE1_HUMAN,Adhesion G protein-coupled receptor E1
UniProtID :Q14246
MASS(da) :97,683
MW(kDa) :65-250 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Mouse
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of Mouse F4/80
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Raw 264.7 cells
Function : Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development..
Tissue specificity :Expression is restricted to eosinophils..
Subcellular locationi :Cell membrane,Multi-pass membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.