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Western blot(SDS PAGE) analysis of extracts from human plasma lysates.Using APOL1 mouse mAb IgG [T49V] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :APOL1,APOL,APOL1_HUMAN,Apolipoprotein L1
UniProtID :O14791
MASS(da) :43,974
MW(kDa) :45
Form :Liquid
Purification :Protein A purification
Host :mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human APOL1.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :human plasma lysates
Function : May play a role in lipid exchange and transport throughout the body. May participate in reverse cholesterol transport from peripheral cells to the liver.
Tissue specificity :Plasma. Found on APOA-I-containing high density lipoprotein (HDL3). Expressed in pancreas, lung, prostate, liver, placenta and spleen.
Subcellular locationi :Secreted.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.