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Western blot(SDS PAGE) analysis of extracts from HeLa cells, serum-starved overnight and then stimulated for 4 hours with TPA..Using Phospho-c-Fos (Ser32)Rabbit mAb IgG [2FEN] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :FOS,G0S7,FOS_HUMAN,Protein c-Fos
UniProtID :P01100
MASS(da) :40,695
MW(kDa) : 62
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser32 of human c-Fos
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells, serum-starved overnight and then stimulated for 4 hours with TPA.
Function : Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation. In growing cells, activates phospholipid synthesis, possibly by activating CDS1 and PI4K2A. This activity requires Tyr-dephosphorylation and association with the endoplasmic reticulum..
Subcellular locationi :Nucleus. Endoplasmic reticulum. Cytoplasm, cytosol.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.