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Phospho-IκBα (Ser32) Rabbit mAb[2637]Cat NO.: A78318

Western blot(SDS PAGE) analysis of extracts from HeLa cells, treated with TNF-α (20 ng/ml,5min)..Using Phospho-IκBα (Ser32)Rabbit mAb IgG [2637] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :NFKBIA,IKBA,MAD3,NFKBI,IKBA_HUMAN,NF-kappa-B inhibitor alpha

UniProtID :P25963

MASS(da) :35,609

MW(kDa) : 40

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser32 of human IκBα

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :HeLa cells, treated with TNF-α (20 ng/ml,5min).

Function : Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and pro-inflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription..

Subcellular locationi :Cytoplasm. Nucleus.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.