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Western blot(SDS PAGE) analysis of extracts from A-431 cells, Forskolin-treated (10 μM, 15 min) ..Using Phospho-VASP (Ser157)Rabbit mAb IgG [EJ37] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :VASP,VASP_HUMAN,Vasodilator-stimulated phosphoprotein
UniProtID :P50552
MASS(da) :39,830
MW(kDa) : 50
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser157 of human VASP
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :A-431 cells, Forskolin-treated (10 μM, 15 min) .
Function : Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping protein. VASP stimulates actin filament elongation by promoting the transfer of profilin-bound actin monomers onto the barbed end of growing actin filaments. Plays a role in actin-based mobility of Listeria monocytogenes in host cells. Regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation..
Tissue specificity :Highly expressed in platelets..
Subcellular locationi :Cytoplasm. Cytoplasm, cytoskeleton. Cell junction, focal adhesion. Cell junction, tight junction. Cell projection, lamellipodium membrane. Cell projection, filopodium membrane.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.