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Western blot(SDS PAGE) analysis of extracts from A-431 cells..Using Phospho-Zyxin (Ser142/143)Rabbit mAb IgG [67AU] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :ZYX,ZYX_HUMAN,Zyxin
UniProtID :Q15942
MASS(da) :61,277
MW(kDa) : 78
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser142 and Ser143 of human Zyxin
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :A-431 cells.
Function : Adhesion plaque protein. Binds alpha-actinin and the CRP protein. Important for targeting TES and ENA/VASP family members to focal adhesions and for the formation of actin-rich structures. May be a component of a signal transduction pathway that mediates adhesion-stimulated changes in gene expression (By similarity)..
Subcellular locationi :Cytoplasm. Cytoplasm, cytoskeleton. Nucleus. Cell junction, focal adhesion.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.