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Western blot(SDS PAGE) analysis of extracts from Raw 264.7 cells.Using CD64/FcγRI Rabbit mAb [8R05] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :Fcgr1,Fcg1,FCGR1_MOUSE,High affinity immunoglobulin gamma Fc receptor I
UniProtID :P26151
MW(kDa) :60-80 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Mouse
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of Mouse CD64/FcγRI
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Raw 264.7 cells
Function : High affinity receptor for the Fc region of immunoglobulins gamma. Functions in both innate and adaptive immune responses..
Tissue specificity :Macrophage-specific.
Subcellular locationi :Cell membrane,Single-pass type I membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.