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Western blot(SDS PAGE) analysis of extracts from Mouse brain tissue lysate.Using HES5 Rabbit mAb IgG [3J02] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :HES5,BHLHB38,HES5_HUMAN,Transcription factor HES-5
UniProtID :Q5TA89
MASS(da) :18,226
MW(kDa) :18kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human HES5.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Mouse brain tissue lysate
Function : Transcriptional repressor of genes that require a bHLH protein for their transcription. Plays an important role as neurogenesis negative regulator (By similarity)..
Tissue specificity :Expressed in fetal heart and brain tumors..
Subcellular locationi :Nucleus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.