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Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using GluCOSe-6-Phosphate Dehydrogenase Rabbit pAb at dilution of 1:1000 incubated at 4℃ over night.
Protein names :G6PD,G6PD_HUMAN,Glucose-6-phosphate 1-dehydrogenase
UniProtID :P11413
MASS(da) :59,257
MW(kDa) :58 kDa
Form :Liquid
Purification :peptide affinity chromatography
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of Human GluCOSe-6-Phosphate Dehydrogenase
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells
Function : Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis..
Tissue specificity :Isoform Long is found in lymphoblasts, granulocytes and sperm.
Subcellular locationi :Cytoplasm, cytosol. Membrane,Peripheral membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.