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Western blot(SDS PAGE) analysis of extracts from Mouse lung tissue lysate.Using Myosin heavy chain Rabbit mAb IgG [B1BB] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :MYH7,MYHCB,MYH7_HUMAN,Myosin-7
UniProtID :P12883/P13533
MASS(da) :223,735
MW(kDa) :224kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Mouse Myosin heavy chain.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Mouse lung tissue lysate
Function : Myosins are actin-based motor molecules with ATPase activity essential for muscle contraction. Forms regular bipolar thick filaments that, together with actin thin filaments, constitute the fundamental contractile unit of skeletal and cardiac muscle..
Tissue specificity :Both wild type and variant Gln-403 are detected in skeletal muscle (at protein level)..
Subcellular locationi :Cytoplasm, myofibril. Cytoplasm, myofibril, sarcomere.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.