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Western blot(SDS PAGE) analysis of extracts from Mouse brain tissue lysate.Using NeuN Rabbit mAb IgG [Y4S7] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :RBFOX3,RFOX3_HUMAN,RNA binding protein fox-1 homolog 3
UniProtID :A6NFN3
MASS(da) :33,873
MW(kDa) :46-55kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human NeuN.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Mouse brain tissue lysate
Function : Pre-mRNA alternative splicing regulator. Regulates alternative splicing of RBFOX2 to enhance the production of mRNA species that are targeted for nonsense-mediated decay (NMD)..
Subcellular locationi :Nucleus. Cytoplasm.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.