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Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using PEPCK/PCK2 Rabbit mAb IgG [G456] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :PCK2,PEPCK2,PCKGM_HUMAN,Phosphoenolpyruvate carboxykinase [GTP], mitochondrial
UniProtID :Q16822
MASS(da) :70,699
MW(kDa) :71KDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human PEPCK/PCK2.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells
Function : Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle..
Subcellular locationi :Mitochondrion.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.