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PEPCK/PCK2 Rabbit mAb[G456]Cat NO.: A71701

Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using PEPCK/PCK2 Rabbit mAb IgG [G456] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :PCK2,PEPCK2,PCKGM_HUMAN,Phosphoenolpyruvate carboxykinase [GTP], mitochondrial

UniProtID :Q16822

MASS(da) :70,699

MW(kDa) :71KDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000,
  • 免疫荧光(ICC/IF) 1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human PEPCK/PCK2.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :HeLa cells

Function : Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle..

Subcellular locationi :Mitochondrion.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.