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Western blot(SDS PAGE) analysis of extracts from Rat testis tissue lysate.Using PGE receptor EP2 (PTGER2) Rabbit mAb IgG [IIME] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :PTGER2,PE2R2_HUMAN,Prostaglandin E2 receptor EP2 subtype
UniProtID :P43116
MASS(da) :39,761
MW(kDa) :52kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human PGE receptor EP2 (PTGER2).
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Rat testis tissue lysate
Function : Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(s) proteins that stimulate adenylate cyclase. The subsequent raise in intracellular cAMP is responsible for the relaxing effect of this receptor on smooth muscle.
Tissue specificity :Placenta and lung.
Subcellular locationi :Cell membrane,Multi-pass membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.