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Western blot(SDS PAGE) analysis of extracts from HepG2 cells.Using EDC4/Ge-1 Rabbit pAb at dilution of 1:1000 incubated at 4℃ over night.
Protein names :EDC4,HEDLS,EDC4_HUMAN,Enhancer of mRNA-decapping protein 4
UniProtID :Q6P2E9
MASS(da) :151,661
MW(kDa) :170 kDa
Form :Liquid
Purification :peptide affinity chromatography
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of Human EDC4/Ge-1
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HepG2 cells
Function : In the process of mRNA degradation, seems to play a role in mRNA decapping. Component of a complex containing DCP2 and DCP1A which functions in decapping of ARE-containing mRNAs. Promotes complex formation between DCP1A and DCP2. Enhances the catalytic activity of DCP2 (in vitro)..
Subcellular locationi :Cytoplasm, P-body. Nucleus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.