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Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using Talin 2 Rabbit mAb IgG [K27W] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :TLN2,KIAA0320,TLN2_HUMAN,Talin-2
UniProtID :Q9Y4G6
MASS(da) :271,613
MW(kDa) :270kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human Talin 2.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells
Function : As a major component of focal adhesion plaques that links integrin to the actin cytoskeleton, may play an important role in cell adhesion. Recruits PIP5K1C to focal adhesion plaques and strongly activates its kinase activity (By similarity)..
Subcellular locationi :Cytoplasm. Cell junction, focal adhesion. Cell junction, synapse. Cell membrane,Peripheral membrane protein,Cytoplasmic side. Cytoplasm, cytoskeleton.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.