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Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using TCTP/TPT1 Rabbit mAb IgG [8Z1F] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :TPT1,TCTP_HUMAN,Translationally-controlled tumor protein
UniProtID :P13693
MASS(da) :19,595
MW(kDa) :23kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human TCTP/TPT1.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells
Function : Involved in calcium binding and microtubule stabilization.
Tissue specificity :Found in several healthy and tumoral cells including erythrocytes, hepatocytes, macrophages, platelets, keratinocytes, erythroleukemia cells, gliomas, melanomas, hepatoblastomas, and lymphomas. It cannot be detected in kidney and renal cell carcinoma (RCC). Expressed in placenta and prostate..
Subcellular locationi :Cytoplasm.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.