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14-3-3 Sigma Mouse mAb[5K43]Cat NO.: A18184

Western blot(SDS PAGE) analysis of extracts from A431 cells.Using 14-3-3 Sigma Mouse mAb IgG [5K43] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :SFN,HME1,1433S_HUMAN,14-3-3 protein sigma

UniProtID :P31947

MASS(da) :27,774

MW(kDa) :30kda

Form :Liquid

Purification :Protein A purification

Host :Mouse

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • 免疫荧光(ICC/IF) 1:100,
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human 14-3-3 Sigma.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :A431 cells

Function : Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53.., p53-regulated inhibitor of G2/M progression..

Tissue specificity :Present mainly in tissues enriched in stratified squamous keratinizing epithelium.

Subcellular locationi :Cytoplasm. Nucleus. Secreted.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.