* 产品详情- */>
Western blot(SDS PAGE) analysis of extracts from A431 cells.Using 14-3-3 Sigma Mouse mAb IgG [5K43] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :SFN,HME1,1433S_HUMAN,14-3-3 protein sigma
UniProtID :P31947
MASS(da) :27,774
MW(kDa) :30kda
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human 14-3-3 Sigma.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :A431 cells
Function : Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53.., p53-regulated inhibitor of G2/M progression..
Tissue specificity :Present mainly in tissues enriched in stratified squamous keratinizing epithelium.
Subcellular locationi :Cytoplasm. Nucleus. Secreted.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.