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Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using AGXT2 Mouse mAb IgG [6B89] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :AGXT2,AGT2,AGT2_HUMAN,Alanine--glyoxylate aminotransferase 2, mitochondrial -3-amino-2-methylpropionate--pyruvate transaminase)
UniProtID :Q9BYV1
MASS(da) :57,156
MW(kDa) :57kDa
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human AGXT2.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells
Function : Can metabolize asymmetric dimethylarginine (ADMA) via transamination to alpha-keto-delta-(NN-dimethylguanidino) valeric acid (DMGV). ADMA is a potent inhibitor of nitric-oxide (NO) synthase, and this activity provides mechanism through which the kidney regulates blood pressure..
Subcellular locationi :Mitochondrion.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.