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Western blot(SDS PAGE) analysis of extracts from human skeletal muscle tissue.Using Alpha Sarcoglycan Mouse mAb IgG [M35L] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :SGCA,ADL,DAG2,SGCA_HUMAN,Alpha-sarcoglycan
UniProtID :Q16586
MASS(da) :42,875
MW(kDa) :50kDa
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human Alpha Sarcoglycan.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :human skeletal muscle tissue
Function : Component of the sarcoglycan complex, a subcomplex of the dystrophin-glycoprotein complex which forms a link between the F-actin cytoskeleton and the extracellular matrix.
Tissue specificity :Most strongly expressed in skeletal muscle. Also expressed in cardiac muscle and, at much lower levels, in lung. In the fetus, most abundant in cardiac muscle and, at lower levels, in lung. Also detected in liver and kidney. Not expressed in brain.
Subcellular locationi :Cell membrane, sarcolemma,Single-pass type I membrane protein. Cytoplasm, cytoskeleton.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.