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Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using CMPK1 Mouse mAb IgG [Y0VM] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :CMPK1,CMK,CMPK,UCK,UMK,UMPK,KCY_HUMAN,UMP-CMP kinase
UniProtID :P30085
MASS(da) :22,222
MW(kDa) :26kda
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human CMPK1.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells
Function : Catalyzes the phosphorylation of pyrimidine nucleoside monophosphates at the expense of ATP. Plays an important role in de novo pyrimidine nucleotide biosynthesis. Has preference for UMP and CMP as phosphate acceptors. Also displays broad nucleoside diphosphate kinase activity..
Tissue specificity :Ubiquitously expressed..
Subcellular locationi :Nucleus. Cytoplasm.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.