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Western blot(SDS PAGE) analysis of extracts from HT-1080 cells.Using Collagen Type V Mouse mAb IgG [YX6Q] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :COL5A1,CO5A1_HUMAN,Collagen alpha-1 chain
UniProtID :P20908
MASS(da) :183,560
MW(kDa) :220kDa
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human Collagen Type V.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HT-1080 cells
Function : Type V collagen is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin.
Subcellular locationi :Secreted, extracellular space, extracellular matrix.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.