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Western blot(SDS PAGE) analysis of extracts from LNCaP cells.Using DHODH Mouse mAb IgG [46B0] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :DHODH,PYRD_HUMAN,Dihydroorotate dehydrogenase , mitochondrial
UniProtID :Q02127
MASS(da) :42,867
MW(kDa) :43kDa
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human DHODH.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :LNCaP cells
Function : Catalyzes the conversion of dihydroorotate to orotate with quinone as electron acceptor. Required for UMP biosynthesis via de novo pathway..
Subcellular locationi :Mitochondrion inner membrane,Single-pass membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.