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Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using DMC1 Mouse mAb IgG [976B] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :DMC1,DMC1H,LIM15,DMC1_HUMAN,Meiotic recombination protein DMC1/LIM15 homolog
UniProtID :Q14565
MASS(da) :37,681
MW(kDa) :37kDa
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human DMC1.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells
Function : Participates in meiotic recombination, specifically in homologous strand assimilation, which is required for the resolution of meiotic double-strand breaks..
Subcellular locationi :Nucleus. Chromosome.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.