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Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using eIF4G2/DAP5 Mouse mAb IgG [8SYW] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :EIF4G2,DAP5,OK/SW-cl.75,IF4G2_HUMAN,Eukaryotic translation initiation factor 4 gamma 2
UniProtID :P78344
MASS(da) :102,362
MW(kDa) :97kDa
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human eIF4G2/DAP5.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells
Function : Appears to play a role in the switch from cap-dependent to IRES-mediated translation during mitosis, apoptosis and viral infection. Cleaved by some caspases and viral proteases..
Tissue specificity :Ubiquitously expressed in all adult tissues examined, with high levels in skeletal muscle and heart. Also expressed in fetal brain, lung, liver and kidney..
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.