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Western blot(SDS PAGE) analysis of extracts from SNB19 cells.Using Na,K-ATPase α1 Rabbit mAb [9G25] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :ATP1A1,AT1A1_HUMAN,Sodium/potassium-transporting ATPase subunit alpha-1 /K ATPase alpha-1 subunit)
UniProtID :P05023
MASS(da) :112,896
MW(kDa) :100 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of Human Na,K-ATPase α1
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :SNB19 cells
Function : This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients..
Subcellular locationi :Basolateral cell membrane,Multi-pass membrane protein. Cell membrane, sarcolemma,Multi-pass membrane protein. Cell projection, axon. Melanosome.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.